Figure 4. No effect of PKA inhibition or activation on CBF responses to changing pH_i during ammonium pre-pulse.

A,simultaneous CBF and pH_i recording from a single, submerged, BCECF-loaded human tracheobronchial epithelial cell pre-treated and continuously perfused with 100 μm H-7 and transiently exposed to 10 mm NH₄Cl. Neither the CBF nor the pH_i responses differed from control cells (see Fig. 2). B, simultaneous CBF and pH_i recording from a single, submerged, BCECF-loaded human tracheobronchial epithelial cell continuously exposed to 10 μm forskolin followed by a transient exposure to 10 mm NH₄Cl.