A, difference (ΔF) images of stage II (E30) VZ waves, showing the spatial extent of the wave (dark regions) in control, picrotoxin (Pic, 100 μm) and neostigmine (NS, 4 μm). The waves were dramatically enhanced by NS, but not by Pic at this stage. B, VZ waves at P3, showing a localized pattern in control, a modest enhancement in the presence of NS (4 μm) and a dramatic enhancement of wave intensity and lateral propagation in the combined presence of NS (4 μm) and Pic (100 μm). All frames were selected to show the typical wavefront size under each recording condition. Scale bar: 0.5 mm.