Figure 6.
Gene expression in tomato after SA or ethylene pretreatment, before and after infection with B. cinerea. Tomato cv Pearson plants were pretreated with either 1 μL L−1 ethylene (E), 4 mm SA (S), or the combination of compounds (se) for 20 h before inoculation with B. cinerea. C, Control treatment. The first set of four lanes (indicated with 0) show gene expression after pretreatment, but before inoculation, whereas the second set of four lanes (indicated with 96) show gene expression after B. cinerea infection at 96 hpi. Bcact, Actin probe of B. cinerea reflecting fungal biomass. Pin I, Proteinase inhibitor I. The panels represent autoradiographs of duplicate blots hybridized with probes as indicated in the left margin.