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. 2004 Aug 26;560(Pt 3):721–736. doi: 10.1113/jphysiol.2004.067801

Figure 4. Pharmacological identification of GlyT1 in Bergmann glia.

Figure 4

A, glycine and sarcosine induced similar inward currents. Both drugs were each applied at 500 μm from a holding potential of −70 mV onto the same cell. B, sarcosine-induced currents (Ba) were reversibly abolished by replacing external Na+ by choline (Bb). C, records of sarcosine-induced currents obtained at different holding potentials from −70 to +50 mV. D, mean I–V curve of sarcosine-induced currents (n = 5). For all of the recordings, sarcosine was pressure applied at 500 μm on cells recorded at a holding potential of −70 mV. E and F, glycine-induced transport currents were markedly and reversibly reduced by bath application of sarcosine (E) and NFPS (F), two blockers of glycine transport through GlyT1. G, bath application of ORG 25543, a GlyT2 inhibitor, did not affect glycine-induced currents.