Figure 2. The effect of H₂O₂ treatment on the viability of C₂C₁₂ myotubes.

A, change in absolute concentration of H₂O₂ with time during incubation in Dulbecco's phosphate-buffered saline. The initial concentration of H₂O₂ was 2 mm. B, viability of 5-day-old cultured myotubes measured at either 4 (□) or 12 (▪) hours following exposure to different concentrations of H₂O₂ for 30 min *P < 0.05 compared to untreated cells; n = 12. C, light microscopy of control untreated 5-day-old myotubes and myotubes at 4 h following treatment with 0.1 or 2 mm H₂O₂ for 30 min.