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. 2004 Sep 9;561(Pt 1):27–37. doi: 10.1113/jphysiol.2004.069922

Figure 3. AMPAR EPSCs precede KAR EPSCs at mossy fibre synapses.

Figure 3

MF-EPSCs recorded in CA3 pyramidal cells at different ages, in control conditions (25 μm d-AP5, and 10 μm bicuculline) and in the presence of GYKI 53655, to isolate KAR-mediated synaptic responses. Left traces, each trace represents the average of 100 traces recorded at a stimulation frequency of 0.5 Hz. Right traces represent average synaptic compound EPSCs in response to a train of 5 stimuli at 50 Hz, at a rate of 0.2 Hz. At P4, and in a proportion of cells at P6, GYKI 53655 abolishes synaptic responses, indicating that MF-EPSC is mediated by AMPAR but not by KARs at early stages of maturation, even in response to a high frequency train of stimuli (5 stimuli at 50 Hz). In some cells between P6 and P9, and in all cells after P10, a small KAR-mediated synaptic component can be detected, with slow kinetics. KAR-EPSCs summate in response to a train of stimuli. Example traces at P6 and at P21.