Figure 7.

Schematic representation of A. acetabulum cell fragments: culture with and without rhizoid and in absence and presence of cytochalasin D. Cells are not drawn to scale; whorls of hairs are omitted. Amputation is indicated by a horizontal line. 1, Intact cell of 30 to 40 mm in length without cap; 2, amputation of apical cell-region only (5) generation of apical- and rhizoid-amputated fragment. The nucleated (3) and enucleated (6) cell fragments were cultured for 7 d in the absence and presence of cytochalasin D. The cultured cellular fragments were then redissected into apical (a), middle (m), and basal (b) fragments (4, 7) and immediately processed for analysis.