Figure 9.

Restablishment of the apical/basal gradient of calmodulin-4 mRNA after decapitation: the role of actin microfilaments. Basal cell fragments (with or without rhizoid) were generated and cultured in the absence or presence of 10 μg mL⁻¹ of cytochalasin D for 7 d after amputation before processing occurred, as described schematically in Figure 7. Experimental conditions are similar to those described in Figure 1; same abbreviations as in Figure 8. The 180-bp RT-PCR product corresponds to calmodulin 4 mRNA and the 850-bp RT-PCR product to 18S rRNA.