Table I.
Survey of the distribution of analyzed mRNAs in the A. acetabulum cell and characterization of the PCR fragments used in the investigation
| Gene Name | mRNA Localization | PCR Fragment Length | No. of Unusual Codons | Function of Protein Product |
|---|---|---|---|---|
| bp | ||||
| UDP-Glc epimerase | Young cells: basalAdult cells: apical | 650 | 2 (TAA/TAG⩵Q) | Conversion of UDP-Glc to UDP-Gal |
| ADP-Glc-pyrophosphorylase | Evenly | 500 | 1 (TAA⩵Q) | Synthesis of activated sugar molecules (ADP-Glc) |
| Actin-1 | Evenly (but accumulates at wounded sites) | 700 | 4 (TAA/TAG⩵Q) | Cytoskeletal protein, involved in plasma streaming |
| Actin-2 | Young cells: evenlyAdult cells: apical | 750 | 1 (TAG⩵Q) | |
| Calmodulin-2 | Basal | 180 | 1 (TAA⩵Q) | Central mediator of Ca2+ signals |
| Calmodulin-4 | Apical | 180 | 2 (TAA/TAG⩵Q) | |
| Ran-G protein | Basal | 160 | 1 (TAG⩵Q) | Transport of RNA and proteins across the nuclear membrane |
| RbcS | Evenly | 250 | 2 (TAA/TAG⩵Q) | Small subunit of Rubisco enzyme |
| 18SrRNA | Evenly | 850 | None | 18S ribosomal rRNA |
| Centrin | Evenly | 300 | 1 (TAA⩵Q) | Ca2+-binding protein (e.g. basal body, centrosome) |
| MAP-kinase | Young cells: basalAdult cells: apical | 300 | 1 (TAA⩵Q) | Ser-Thr kinase, transduction of extracellular signals to intracellular targets |
| α- and β-Tubulin | Evenly | α: 650β: 750 | Not applicable | Cytoskeletal proteins |