Table 1.

MCT1 and 4 protein and mRNA in rat hindlimb muscles (mean ±s.e.m.)

Muscle	MCT1 protein (a.u./20 μg potein)	MCT4 protein (a.u./20 μg potein)	MCT1 mRNA (a.u./18S rRNA)	MCT4 mRNA (a.u./18S rRNA)
Soleus	311 ± 36**	100 ± 8.5	ND	ND
Red gastrocnemius	238 ± 27*	298 ± 9.1***	100 ± 2.3*	100 ± 5.0
White gastrocnemius	100 ± 3	315 ± 7.8***	85 ± 2.3	97.8 ± 6.1

Equal quantities of protein (20 μg) and RNA (3 μg) were loaded for each muscle so as to be able to make direct comparisons among muscles. Protein data are arbitrary units (a.u) per 20 μg protein, with the mean for muscles with the lowest content of MCT1 (white gastrocnemius) and MCT4 proteins (soleus) being set to 100. mRNA data are normalized to 18S rRNA, followed by a further transformation of the data so that the red gastrocnemius muscle ratio ± s.e.m. was set to 100 ± s.e.m., with the white gastrocnemius being set relative to this. ND, not determined. MCT1, n = 13; MCT4, n = 7; MCT1 mRNA, n = 3; MCT4 mRNA n = 3.

^*MCT1: RG versus WG, P < 0.05

^**MCT1: SOL versus RG, and SOL versus WG P < 0.05

^***MCT4: RG versus SOL and WG versus SOL, P < 0.05.