Table 6.
Comparison of electrophysiological properties of MCs at 34°C
| Layer V (n = 25) | Layer VI (n = 7) | |
|---|---|---|
| AP1 waveform | ||
| AP ampl., av (mV) | 54.97 ± 12.29 | 52.57 ± 9.22 |
| AP duration, av (ms) | 3.45 ± 0.83† | 2.97 ± 0.24 |
| AP duration HW, av (ms) | 1.57 ± 0.33 | 1.44 ± 0.36 |
| AP rise time, av (ms) | 1.19 ± 0.22 | 1.11 ± 0.16 |
| AP fall time, av (ms) | 2.26 ± 0.62† | 1.86 ± 0.09 |
| AP rise rate (mV ms−1) | 47.20 ± 11.11 | 48.69 ± 12.16 |
| AP fall rate (mV ms−1) | −27.74 ± 7.13 | −33.19 ± 10.31 |
| AHP fast, av (mV) | 7.34 ± 11.92 | 13.40 ± 7.28 |
| AP2 waveform | ||
| AP ampl., av (mV) | 52.06 ± 8.97 | 55.55 ± 11.13 |
| AP duration, av (ms) | 3.72 ± 0.79 | 3.36 ± 0.19 |
| AP duration HW, av (ms) | 1.75 ± 0.36 | 1.66 ± 0.32 |
| AP rise time, av (ms) | 1.32 ± 0.26 | 1.25 ± 0.19 |
| AP fall time, av (ms) | 2.41 ± 0.57† | 2.11 ± 0.09 |
| AP rise rate (mV ms−1) | 40.77 ± 9.35 | 45.98 ± 13.14 |
| AP fall rate (mV ms−1) | −24.45 ± 5.77 | −29.91 ± 8.35 |
| AHP fast_av (mV) | 10.84 ± 3.44 | 13.24 ± 5.05 |
| AP1/AP2 waveform | ||
| Ch AP ampl. (%) | −3.14 ± 10.69 | 4.80 ± 4.44 |
| Ch AP duration (%) | 10.01 ± 4.11 | 11.66 ± 2.94 |
| Ch AP duration HW (%) | 12.42 ± 5.18 | 13.63 ± 4.23 |
| Ch AP rise rate (%) | −17.16 ± 19.69 | −7.18 ± 5.64 |
| Ch AP fall rate (%) | −14.84 ± 16.80 | −9.89 ± 6.05 |
| Ch AHP fast (%) | 15.13 ± 59.00 | 10.22 ± 40.89 |
| IV | ||
| Input resistance for peak (MΩ) | 326.21 ± 196.53 | 228.68 ± 119.89 |
| Input resistance for SS (MΩ) | 254.44 ± 166.89 | 171.50 ± 81.20 |
| Rectification index for peak IV | 21.79 ± 10.71 | 18.47 ± 2.27 |
| Rectification index for SS IV | 16.70 ± 6.26 | 14.31 ± 2.97 |
| Maximum Sag is (mV) | 4.44 ± 4.17 | 5.37 ± 2.78 |
| Delta | ||
| Delta av decay time constant (ms) | 17.57 ± 9.24 | 34.05 ± 27.38 |
| AP threshhold | ||
| AP threshold (mV) | −46.09 ± 5.54 | −43.25 ± 1.15 |
| AHP after 1st AP in the ramp | 7.49 ± 3.40 | 11.83 ± 5.83 |
| sAHP | ||
| sAHP max (mV) | 2.61 ± 2.61 | 6.55 ± 7.15 |
| sAHP τ (ms) | 1693.90 ± 1227.04 | − |
| Discharge | ||
| Slope of ID threshold | 0.15 ± 0.06 | 0.12 ± 0.10 |
| Av delay to 1st spike | 0.06 ± 0.03 | 0.05 ± 0.03 |
| SD for delay to 1st spike | 0.05 ± 0.05 | 0.05 ± 0.03 |
| Av delay to 2nd spike | 0.06 ± 0.03 | 0.05 ± 0.01 |
| SD for delay to 2nd spike | 0.04 ± 0.02 | 0.06 ± 0.04 |
| Av initial burst interval | 0.05 ± 0.03 | 0.05 ± 0.02 |
| SD for av initial burst interval | 0.04 ± 0.03 | −25.43 ± 56.99 |
| Av initial accom | −44.74 ± 35.67 | −95.61 ± 102.10 |
| Av SS accom | −212.19 ± 172.70 | −89.00 ± 68.49 |
| Rate of accom ItoSt | −19.10 ± 16.58 | −23.74 ± 34.75 |
| Av accom at SS | −30.54 ± 58.30 | −5.62 ± 9.95 |
| Av rate accom during SS | −3.43 ± 7.22 | −0.09 ± 1.84 |
| Av CV discharge | 3.32 ± 1.76 | 2.93 ± 2.15 |
| Av skew discharge | 0.13 ± 1.45 | −0.45 ± 0.67 |
| Av discharge STUT | 0.15 ± 0.12 | 12.30 ± 27.33 |
†
P < 0.05, comparison between different layers.