Figure 5. Effects on sIPSCs of pretreatment with concanamaycin, Ca²⁺-free solution containing 5 mm EGTA, or pretreatment with thapsigargin.

A and B, effect of concanamycin pretreatment (Conc, 0.5 μm for at least 2 h, 5 cells), Ca²⁺-free solution containing 5 mm EGTA (5 cells) and thapsigargin pretreatment (Thap, 3 μm for 1 h, 5 cells) on the sIPSC frequency (A) and amplitude (B) in non-ischaemic solution. P values are compared with control conditions. C–E, data in ischaemia solution. C, sample traces at −33 mV showing sIPSCs, taken 4 min after the start of the ischaemic episode after concanamycin pretreatment, in Ca²⁺-free/5 mm EGTA solution, and after thapsigargin pretreatment. Average data from 5 cells for frequency (D) and amplitude (E) of sIPSCs during ischaemia; points represent 30 s bins of events. Control data (Con) from Fig. 3 are superimposed for comparison. Amplitude data are not shown for Ca²⁺-free conditions or concanamycin because of the small number of events measured.