Table 3.
Commonly used high fat feeding protocols
| Diet | Protein | Starch | Simple sugars | Fibre | Lipid | Vitamins | Minerals | Methionine | Reference |
|---|---|---|---|---|---|---|---|---|---|
| HVF 40% | Casein 15% | 15% | Sucrose 15% | 5% | Veg oil 40% | (AIN-76) 1% | (AIN-76) 3.5% | NA | Guo & Jen (1995) |
| C 4% | Crude 23.4% | 15% | Sucrose 15% | 5% | Corn oil 5.3% | (AIN-76) 1% | (AIN-76) 3.5% | NA | Guo & Jen (1995) |
| HAF 24% | Crude 19.5% | 35.8% | Sucrose 5.4% | 4.5% | Corn oil 4.3%, Lard 20% | R/M 3 Premix 2.6% | R/M 3 Premix 2.6% | 0.2% | Khan et al. (2003) |
| C 4% | Crude 21.6% | 48.8% | Sucrose 5.4% | 4.5% | Corn oil 5.3% | R/M 3 Premix 1.6% | R/M 3 Premix 1.6% | 0.2% | Khan et al. (2003) |
| HSF 17.4% | Crude 15.4% | — | 43.3% | 5% | Coconut oil 17.4% | (AIN-93G) 3% | (AIN-93) 1.1% | NA | Siemelink et al. (2002) |
| HFF 18.1% | Crude 15.5% | — | 44.5% | 5% | Menhaden oil 18.1% | (AIN-93G) 3% | (AIN-93) 1.1% | NA | Siemelink et al. (2002) |
| HUF 18.4% | Crude 15.8% | — | 42.5% | 5% | Soy oil 18.4% | (AIN-93G) 3% | (AIN-93) 1.1% | NA | Siemelink et al. (2002) |
| HAF22% | 19% (88% casein) | 34% | — | 17.9% | Corn oil 1.9%, Lard 20.1% | R/M 3 Premix 5.01% | R/M 3 Premix 5.01% | 0.4 | Holness & Sugden (1999) |
| C 4% | 22% (88% casein) | 8% | Cerelose 55.1% | 5% | Soy oil 4.3% | R/M 3 Premix 5.05% | R/M 3 Premix 5.05% | 0.2 | Holness & Sugden (1999) |
All percentage values are given as w/w. *Total vitamin/mineral mix is 3.5%. Note that due to varying moisture and nitrogen free extract (polysaccharide) content, rows will not sum to 100%. Abbreviations: HVF, vegetable oil based high fat diet; HAF, high animal fat diet; HFS, high saturated fat diet; HFF, high fish oil diet; HUF, high unsaturated fat diet; NA, data not available from reference. Note that although vitamin and mineral pre-mixes vary subtly, all are similar to the AIN-76 formulation.