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. 2004 Oct 7;561(Pt 2):547–557. doi: 10.1113/jphysiol.2004.075168

Figure 4. Whole-cell patch-clamp measurements of hKCNE2/hKCNQ1-transfected COS cells (A and B) and mouse parietal cells (C).

Figure 4

Cells were clamped stepwise from –95 to +25 mV. A, effect of forskolin (FSK) on a non-transfected cell (whole-cell current normalized to cell capacitance; left upper panel) and a KCNE2/KCNQ1 co-transfected cell (right upper panel). Left lower panel shows the summary of the FSK effect on whole-cell current in KCNE2/KCNQ1 co-transfected cells. Note the shift of reversal potential of the whole-cell current indicating FSK-induced hyperpolarization of the cells. Right lower panel shows the effect of FSK on the 293B-sensitive current as a measure of KCNE2/KCNQ1-specific current. B, effect of ATP on whole-cell current and 293B-sensitve current. Arrangement of panels as described for A. C, effect of 293B on whole-cell current of freshly isolated mouse parietal cells in the continuous presence of forskolin (5 μm) plus carbachol (100 μm). Error bars: s.e.m. Stars: significantly different from control (con). Number of experiments shown in parentheses.