A, time course for the effect of 500 nm angiotensin II (bar) on the membrane potential of pericytes (n = 6) located on freshly isolated microvessels. Voltage was sampled at 50 ms intervals; the black line shows the means and the vertical grey lines the s.e.m.s. B, current traces before angiotensin II exposure (Control), 15–60 s after the onset of exposure to 500 nm angiotensin II, and approximately 5 min after washout of angiotensin II. The clamp protocol is shown below. C, I–V plots of the steady-state currents shown in B. Control (▪); Angiotensin II (•); Recovery (◊). D, mean angiotensin-induced current (difference between angiotensin and control) recorded in nine pericytes. E, time course for the effect of 500 nm angiotensin II (bar) on the current recorded in pericytes (n = 7) clamped at −58 mV and sampled at 50 ms intervals. To facilitate determination of the mean angiotensin-induced current, the current record was adjusted for each to the seven sampled pericytes so that at 2 min prior to exposure to angiotensin II, the current amplitude was 0 pA. The black line shows the means and the vertical grey lines the s.e.m.s of the adjusted current records.