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. 2004 Oct 14;561(Pt 3):777–791. doi: 10.1113/jphysiol.2004.070631

Figure 3. Two-dimensional gel analyses of NTG and TG α-TM276 myofibrillar proteins.

Figure 3

Total myofibrillar proteins from both NTG (A) and TG α-TM276 (B and C, line 5 and 6, respectively) hearts were subjected to reduced reduced two-dimensional electrophoresis and transferred to nitrocellulose membranes, which were then probed with a striated muscle-specific TM antibody, CH-1. pI values (4.7–5.9) are indicated at the top. The TG samples contain both α-TM276 and endogenous TM spots while the NTG sample contains only endogenous α-TM.