Figure 1.

(A) Analysis of purified inclusion bodies by SDS/PAGE and Coomassie staining. Two microliters of HLA-A1 (lane 1) or β2m (lane 2) inclusion bodies in freezing buffer were loaded on the gel. The expected size of the full-length heavy chain product is indicated with an arrow. (B) Gel filtration profile of the folding mixture, after an incubation of 36 h at 4°C. The mixture was concentrated by ultracentrifugation and loaded on a Superdex 200 column. The shoulder after peak 3 is a component of the folding buffer. (C) SDS/PAGE analysis and Coomassie staining of the peaks obtained by gel filtration. Lanes 1–3 correspond to 20 μl of fractions from peaks 1–3, respectively.