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Labeling EBV-transformed B cells with phage-antibody fd-Fab-G8. Cells were incubated with 100 μM peptide MAGE-1-A1 (thick lines) or MAGE-3-A1 (thin lines), and labeled with fd-Fab-G8 or a control fd phage. Bound phages were detected by sequential incubations with polyclonal goat anti-fd antibodies and rabbit anti-goat Ig antibodies coupled to fluorescein.
