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Labeling melanoma cell lines with phage-antibody fd-Fab-G8. Melanoma cells MZ2-MEL 3.0 (HLA-A1+, MAGE-A1+) or MZ2-MEL 2.2 (HLA-A1+, MAGE-A1−) were incubated with phage antibodies fd-Fab-G8 (thick lines) or control phage antibodies (thin lines). Bound phages were detected by sequential incubations with polyclonal goat anti-fd antibodies and rabbit anti-goat Ig antibodies coupled to fluorescein.
