Figure 1.

Gene structure of the patatin-related AtPLA-family in Arabidopsis and a phylogenetic tree of several patatin-related plant PLA and animal iPLA₂ sequences. A, Gene structure of the AtPLA family in Arabidopsis. Exons are symbolized by gray boxes, those containing the LRR by black boxes, the catalytic center by white boxes, and introns by black lines. cDNA sequences described in this work are marked by an asterisk. The sequences are deposited in database as follows: AtPLA I (accession no. AC004392), AtPLA IIA (accession no. AC002505), AtPLA IIB (accession no. AC004697), AtPLA IIIA (accession no. AL049655.1), AtPLA IIIB (accession no. AL138648), AtPLA IVA (db_xref GI:4006869), AtPLA IVB (db_xref GI:4006870), AtPLA IVC (db_xref GI:4006871), AtPLA IVD (accession no. AL050352.1), and AtPLA V (accession no. AB016875.1). A 500-bp size standard is indicated. B, Phylogenetic tree produced by the program ClustalW (http://www2.ebi.ac.uk/clustalw/) on the amino acid structures of the AtPLA gene-family, a putative bacterial protein from Anabeana sp. (accession no. AJ269505.1; Rouhiainen et al., 2000), a putative protein from the nematode Caenorhabditis elegans (accession no. AC084197.1), a putative protein from the fruitfly (Drosophila melanogaster; accession no. AE003550.2), an iPLA₂ from human (accession no. JC7284; Tanaka et al., 2000), and a patatin class I precursor from potato (accession no. P11768; Mignery et al., 1988) displayed by the program TREEVIEW (Page, 1996).