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. 2002 Sep;130(1):111–119. doi: 10.1104/pp.005561

Figure 1.

Figure 1

Northern-blot analysis of barley Pbf expression during germination. A, Ten micrograms of total RNA from different tissues of 2-d germinated kernels of the barley cv Bomi: shoots (S), roots (R), scutellum (Sc), and aleurone (A). For comparison, a sample of 4 μg of total RNA from developing endosperms (Dv/E) at 15 d after pollination (DAP) was included. B, Comparative accumulation of Pbf transcripts in germinating barley aleurones. Total RNA (10 μg) was extracted from aleurones rehydrated during 0, 16, 24, 48, and 96 h. The blot was first hybridized with the barley Pbf-specific probe (BPBF) and subsequently with a probe for the gene Al21 of the cathepsin-B type from wheat (Thiol protease) and/or a 18S rRNA probe as a loading control.