Figure 5.

Schematic structure of effector and reporter constructs. A, Schematic representation of the effector constructs. The barley Pbf (BPBF protein) and GAMyb genes were under the control of the cauliflower mosaic virus (CaMV) 35S promoter (p35S) followed by the first intron of the maize AdhI gene (I-ADHI) and, downstream, flanked by the 3′-nopaline synthase (nos) sequences (nos). B, Diagrammatic structures of the reporter constructs used in this study: pCBG13, pCBG13.1, and pCBG13.5 as described in Cejudo et al. (1992a). The white and black boxes indicate, respectively, the location of putative binding sites for BPBF (D₁, D₂, and D₃) and GAMYB (M) transcription factors, along with the cathepsin B-like thiol protease AL21 promoter sequences. C, Sequence of cis-motifs conforming the putative DOF- and MYB-binding sites in the AL21 gene promoter. Numbers at the left indicate the position from the translation start codon. Motifs containing the BPBF-binding sequence core (indicated by uppercase letters) are shown aligned with the consensus sequence for pyrimidine box elements.