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F 500 does not significantly induce the accumulation of PR-1 proteins in tobacco leaves. One-half of a leaf of a tobacco plant was infiltrated with 0.5 mm F 500, whereas the other one-half was infiltrated with 0.5 mm SA (positive control). At the indicated time points post treatment, leaf tissue was assayed for the accumulation of PR-1 proteins by western-blotting analysis.
