Figure 4. Effects of dDAVP on I_scon confluent cultures of wild-type and Scnn1b mutant CCD cells.

I_sc was measured in sets of confluent +/+^(L), L/+, L/L, +/+^(m) and m/m CCD cells grown on filters before (○) and after adding amiloride (10⁻⁵m, ▴) or sequential additions of 10⁻⁸m dDAVP (•) and amiloride (▴). Amiloride was added to the apical side of the filters, whereas the dDAVP was added to the basal side of the filters. The bars represent Ams I_sc values from untreated (open bars) and dDAVP-treated cells (filled bars) after adding 10⁻⁵m amiloride. Values are means ± s.e.m. from five to ten separate filters in each condition tested. **P < 0.01versus untreated +/+ values.