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Isc was measured in sets of confluent cultures of +/+(L), L/L, +/+(m) and m/m CCD cells grown on filters and sequentially incubated without (^) or with apical amiloride (Am, 10−5m), basal dDAVP (10−8m, •) and apical NPPB (10−4m, ▪). Bars represent the relative ΔIsc increase caused by dDAVP. Values are means ± s.e.m. from five to seven separate experiments for each condition tested. *P < 0.05 between groups.
