Table 1.

Electrophysiological parameters of primary cultured wild-type and Scnn1b mutant CCD cells

CCD	(n)	RT (Ω cm2)	PD (mV)	Isc (μA cm−2)
+/+(L)	(51)	1895 ± 93	− 30.3 ± 1.8	16.6 ± 1.8
L/+	(42)	1488 ± 47b	− 56.9 ± 1.8b	39.3 ± 1.0a
L/L	(41)	1352 ± 44b, c	− 80.4 ± 3.7b, d	69.0 ± 6.7b, d
+/+(m)	(30)	1962 ± 100	− 32.4 ± 2.5	17.0 ± 0.6
m/m	(31)	2017 ± 240	− 5.7 ± 0.9b	3.2 ± 0.4b

Isolated CCDs were seeded and gn on Transwell filters as described in the Methods section. The transwwepithelial electrical resistance (R_T), voltage (P_D), and short-circuit current (I_sc) were measured under basal conditions in confluent CCD cells dissected from L/+, L/L and m/m mice and the corresponding wild-type +/+^(L) and +/+^(m) littermates. Values are means ± s.e.m. from (n) individual measurements.

^aP < 0.01,

^bP < 0.001versus wild-type +/+ values;

^cP < 0.05,

^dP < 0.01versus L/+ values.