Figure 6. Partial attenuation of rhythmic activity in the rat substantia gelatinosa in vitro by the gap junction uncoupling agents carbenoxolone and octanol.

A, field recording of potassium-induced rhythmic activity before (left panel) and after 45 min 100 μm carbenoxolone (middle panel). B, carbenoxolone reversibly reduced the power of the spectrum (middle panel), without affecting frequency (C). D, quantified data (n = 6) reveal that carbenoxolone reduces the peak power and area with no significant effect on the frequency of activity. A similar profile was obtained with octanol (45 min, 1 mm), another gap junction uncoupler (n = 6). *P < 0.05 paired t test versus control.