Figure 3. GsMTx4 blocks stretch-activated channels in outside-out patches from mdx mouse myotubes.

Pressure step (A) applied for 500 ms every 2 s, to an outside-out patch from an mdx mouse myotube held at –60 mV, activates stretch-activated channels with a unitary current of 1.8 pA (B). Four representative records are shown prior to GsMTx4 application. Multiple steps are averaged (C) to produce the average patch current records in the absence (□, n = 11 pressure steps) and presence of GsMTx4 (▄, n = 11 pressure steps), showing that the peptide blocks these currents.