Table 1.
Hybridoma | Total no. | Allele | No. with rearrangements
|
|||
---|---|---|---|---|---|---|
GL | DJ | VD | V(D)J | |||
Jβ1ω/ω | 50 | ω | 0 | 34 | 0 | 16 |
ω | 0 | 0 | 0 | 50 | ||
Jβ1M2/ω | 106 | ω | 0 | 1 | 0 | 105 |
M2 | 83 | 0 | 22 | 1 |
TCRβ allele configuration in T cell hybridomas was determined by Southern blot analysis of HindIII- or EcoRI-digested genomic DNA by using probes C, D, and E (Fig. 3A). GL, nonrearranged.