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. 2000 Jun 27;97(14):7975–7980. doi: 10.1073/pnas.130190597

Table 1.

Analysis of TCRβ rearrangements in Jβ1ω/ω and Jβ1M2/ω αβ T cell hybridomas

Hybridoma Total no. Allele No. with rearrangements
GL DJ VD V(D)J
Jβ1ω/ω 50 ω 0 34 0 16
ω 0 0 0 50
Jβ1M2/ω 106 ω 0 1 0 105
M2 83 0 22 1

TCRβ allele configuration in T cell hybridomas was determined by Southern blot analysis of HindIII- or EcoRI-digested genomic DNA by using probes C, D, and E (Fig. 3A). GL, nonrearranged.