Table 1.
INBC (pA pF−1) | volume (pl) | JNBCe (mm min−1) | JtotNBC (mm min−1) | JNBCe/JtotNBC | |
---|---|---|---|---|---|
Rat | 0.04 | 18a | 0.40 | 0.68 | 0.6 |
(pHi 7.05) | 32b | 0.22 | 0.68 | 0.3 | |
44c | 0.16 | 0.68 | 0.2 | ||
Rabbit | 0.04 | 29d | 0.28 | 0.42 | 0.7 |
(pHi 7.00) | 31e | 0.26 | 0.42 | 0.6 | |
38f | 0.22 | 0.42 | 0.5 | ||
Guinea pig | 0.02 | 18a | 0.18 | 0.32 | 0.6 |
(pHi 7.07) | 46c | 0.08 | 0.32 | 0.3 | |
28g | 0.12 | 0.32 | 0.4 |
It was assumed that electrogenic NBC had a 1 : 2 stoichiometry and JNBCe (mm min−1 l−1) was given as: JNBCe= 2(INBC× Cm)/(F× cell volume), where Cm is membrane capacitance (pF) and F is the Faraday constant. Values for Cm were 149 pF (rat), 175 pF (rabbit) and 155 pF (guinea pig). Values of INBC at −85 mV were taken from Fig. 7C and those for JtotNBC at the same pHi were taken from Fig. 2A. Surface/volume ratios (μm2μm−3) were:
0.84 (Satoh et al. 1996),
0.46 (Page, 1978),
0.34 (Page et al. 1971),
0.60 (Page & Surdyk-Droske, 1979),
0.56 (Page, 1978),
0.46 (Satoh et al. 1996).
Fixed volume of 28 pl (Campbell et al. 1987).