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. 2004 Nov 25;562(Pt 3):673–685. doi: 10.1113/jphysiol.2004.077685

Figure 9. Loss and replacement of AChRs from endplates.

Figure 9

Four days after electroporation of rapsyn–EGFP plasmid, the surface of the TA muscle was again exposed, and AChRs were saturated with TRITC-α-BGT. A and C, on day 8 after electroporation (4 days after TRITC-α-BGT incubation), endplates negative (A) and positive (C) for rapsyn–EGFP were imaged to reveal AChRs retained from the time of TRITC-α-BGT-labelling 4 days earlier (Residual AChR). The same endplates were imaged again after re-labelling with TRITC-α-BGT to reveal total AChR (B and D). E, quantitative analysis of TRITC-α-BGT fluorescence at endplates (as shown in A–D). Residual AChR and added AChR (additional AChR revealed by re-labelling with TRITC-α-BGT) were normalized to total TRITC-α-BGT intensity. Rapsyn–EGFP-positive endplates showed higher levels of residual AChR and lower levels of replacement (Added) AChRs compared with rapsyn–EGFP-negative endplates (*P < 0.05; n = 7 for each group). Scale bar, 10 μm.