Figure 3. Effects of low-Na⁺ diet on AT₁ and AT₂ receptor mRNA and protein expression in tissues from non-pregnant and pregnant rats.

Data for mRNA are expressed as AT₁ or AT₂/β-actin ratio (arbitrary units) and results shown (A and C) represent means ± s.e.m. from five separate experiments. Membrane proteins were prepared and photoaffinity labelling performed as described in Methods. Data for receptor protein were compared to those from normal non-pregnant controls and results shown (B and D) represent means ± s.e.m. from three separate experiments. Significantly different from values obtained for non-pregnant rats fed a normal diet indicated by ^aP < 0.05, ^bP < 0.01. Significantly different from values obtained for pregnant animals on a normal diet indicated by *P < 0.05. T.N.A., tissues not available. N.D., not detected.