Figure 10. Pharmacological modulation of glucose triggered [Ca²⁺]_i responses.

A, cytoplasmic Ca²⁺ concentration ([Ca²⁺]_i) in a single cell in response to 10 mm glucose in the absence or presence of 5 μm nifedipine + 1 μmω-conotoxine GVIA or 0.3 μm TTX as indicated. B, mean [Ca²⁺]_i measured during a 30-s period during the application of 10 mm glucose before (filled bars), during (unfilled bars) and after (filled bars) addition of 0.3 μm TTX, 5 μm nifedipine or 5 μm nifedipine +1 μmω-conotoxin GVIA. The number of cells is indicated above the bars. Error bars represent 1 s.e.m. and significance was tested using Student's t test comparing the response to 10 mm glucose in the presence of drugs to the mean response before and after drug application: n.s = non significant, ***P < 0.001.