. 2004 Dec 20;563(Pt 1):193–201. doi: 10.1113/jphysiol.2004.078642

Table 2.

¹²⁵I-labelled angiotensin II (Ang II) binding characteristics of glomeruli from control and low-protein (LP) rats

	Control	LP
B_max
¹²⁵I-Ang II	291.6 ± 27.4	445.7 ± 27.4^†
¹²⁵I-Ang II and losartan	44.4 ± 5.89	26.3 ± 3.85^*
¹²⁵I-Ang II and PD123319	217.4 ± 16.3	370.5 ± 19.7^‡
K_D
¹²⁵I-Ang II	2.87 ± 0.85	0.84 ± 0.20
¹²⁵I-Ang II and losartan	2.45 ± 1.06	0.74 ± 0.45
¹²⁵I-Ang II and PD123319	2.19 ± 0.55	0.73 ± 0.16^*

The binding constant (B_max, expressed in femtomoles per milligram glomerular protein) and binding affinity (K_D, expressed in picomoles of ¹²⁵I-Ang II) are shown for ¹²⁵I-Ang II alone (10⁻¹⁰–10⁻⁷m, control n = 9 from 6 litters, LP n = 7 from 6 litters), ¹²⁵I-Ang II plus the AT₁ receptor antagonist losartan (10⁻⁴m, control n = 7 from 5 litters, LP n = 7 from 5 litters) and ¹²⁵I-Ang II plus the AT₂ receptor antagonist PD123319 (10⁻⁴m, control n = 7 from 5 litters, LP n = 7 from 5 litters). Data are presented as means ±s.e.m. Statistical comparisons were by one-way ANOVA and Duncan's test. Differences between control and LP glomeruli treated in the same manner are shown

(P < 0.05,

^†

P < 0.01,

^‡

P < 0.001); for comparisons between different treatments see the results text.