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Effects of aCSF and different doses of bicuculline microdialysed into the PF-LHA during the lights-on period (ZT5–ZT8) on sleep–wake behaviour of rats during the 2-h recording period before they were killed. As compared to aCSF treatment, bicuculline induced a significant increase in active waking (A), and suppressed both non-REM (C) and REM sleep (D). **P < 0.01; *P < 0.05 level of significance (independent t test).
