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Effects of 20 μm bicuculline on mean percentage change in HCRT+/Fos+versus MCH+/Fos+ neurones during the lights-on period (A) and during waking in the lights-off (B) periods. The percentages of HCRT+/Fos+ or MCH+/Fos+ neurones on the contralateral side are the mean of neurones found in grids equivalent to the first three ipsilateral grids. **P < 0.01 level of significance (independent t test).
