Figure 2. G_i/o-protein dependence of I_bacl.

I_bacl is not inducible in cultured Purkinje cells pretreated with pertussis toxin (PTX), a G_i/o-protein inhibitor (0.5 μg ml⁻¹, ≥20 h). A, sample voltage-ramp-activated currents recorded from a cell consecutively before (Basal) and at the end of (Baclofen) a 2 min application of 3 μm baclofen. E_K =−57.6 mV. B, mean amplitude at −130 mV of I_bacl (baclofen, 3 μm, 2 min) measured in the untreated cells (n = 12, reproduced from Fig 1E, −) and the PTX-pretreated cells (n = 7, +). Inward deflection is taken as positive. Error bars, ± s.e.m.**P < 0.01, Student's unpaired t test.