Figure 3.

PLC is not involved in ABA-induced RAB18 expression in Arabidopsis suspension cells. A, Relative amount of PA extracted from cells preincubated for 2 h with ³³P and treated with ABA (10⁻⁵ m) or ABA-BSA (10⁻⁵ m equivalent ABA) for 30 min. Bars, sd, n = 3. B, Northern-blot analysis of total RNA (10 μg) extracted from cells treated for 3 h with ABA-BSA (10⁻⁵ m equivalent ABA) and neomycin (50 μm), U73122 (10 μm), or U73343 (10 μm). Ethidium bromide staining of rRNAs is shown as control.