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. 2005 Jan 13;563(Pt 2):345–358. doi: 10.1113/jphysiol.2004.080028

Figure 1. Activation of NR1/NR2A or NR1/NR2B channels in outside-out patches in response to a synaptic-like brief pulse of saturating glutamate.

Figure 1

A and B, patches with only a single active NR1/NR2A or NR1/NR2B channel were exposed to a brief (3–8 ms) pulse of 1 mm glutamate in the continued presence of 50 μm glycine. The tip current used to determine the time course of solution exchange is plotted on the top trace. Twenty representative current responses from one patch are displayed for NR1/NR2A or NR1/NR2B channels (resampled at 2.5 kHz and filtered at 1 kHz for display; V =−80 mV for NR1/NR2A, −100 mV for NR1/NR2B). The mean current response for each patch was normalized to the unitary channel current to convert the waveform to an absolute open probability. This open probability waveform was averaged among six patches and the resulting composite open probability waveform is displayed below the individual current traces. The distribution of activation durations, defined as the total time between and including the first and last channel opening, is shown as an inset adjacent to the open probability mean waveform.