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Effect of time of VP32947 addition on inhibition of BVDV growth. VP32947 was added to BVDV-infected MDBK cell cultures to a final concentration of 167 nM at the indicated times postinfection. For the “minus 1 h” time point, VP32947 was included during the 1-h virus adsorption period. All cultures were harvested at 14 h postinfection, and virus yield was quantified by plaque assay. The data are presented as the percent of virus produced in the absence of drug.
