Figure 4.

Effect of VP32947 on viral RNA synthesis in F224S DR virus-infected cells. (A) Viral RNA synthesis was determined in cell cultures in the absence (○) or presence (●) of 167 nM VP32947 as described in Materials and Methods section. (B) MDBK cells were mock-infected or were infected with either WT or F224S DR virus. After 6 h, actinomycin D was added to 5 μg/ml without (−) or with (+) 167 nM VP32947. After an additional 2 h, ³H-uridine (Amersham) was added to the cultures. Cells were harvested 3 h later, and total RNA was extracted and subjected to urea-agarose gel electrophoresis followed by fluorography (21).