Figure 6. Parameter (α and V_1/2) variation for all mutations.

Reference is the average value of the given parameter for the entire control population (n = 211; ±s.d.) for the control population. A, variation of α with mutation. Note that S238D had much higher α value (45.5 ± 5) than other mutations. B, variation of V_1/2 with mutation. Several of the mutations present significant shifts. The shift for the double mutation seems to be the accumulation of shifts from the corresponding single mutations. The C1 mutation had been excluded from the V_1/2 graph, as it displayed a very large shift (−134 ± 6 mV). We do not show charge density in a separate panel, because all values were within the range of ± 1 standard deviation for the wild-type population.