Figure 3. NMNAT Is Highly Enriched in the Nervous System and the Muscle Nuclei.

(A–C) Third instar larval neuromuscular junction (NMJ) immunolabeled for HRP (red), with nc82 to mark the active zones (green), and for NMNAT (blue). In the NMJ, NMNAT appears as punctae that co-localize with nc82. NMNAT is also enriched in the muscle nucleus (marked by N in [A]).

(D–F) Optic lobe (P+60%) immunolabeled for Synaptotagmin (syt) to mark synaptic vesicles (blue), with nc82 to mark active zones (green). and for NMNAT (red). NMNAT is highly enriched in the photoreceptor cell body in the eye and in the cell bodies of different types of neurons in the lamina cortex and medulla cortex, but is present at lower levels in lamina. la, lamina; lc, lamina cortex; mc, medulla cortex; me, medulla.

(G–I) MARCM analysis of adult lamina. GFP marks the mutant patches. Laminae are immunolabeled with nc82 to mark photoreceptors (blue) and for NMNAT (red). NMNAT labeling appears as a punctate pattern decorating nc82 labeling, suggesting that NMNAT is present in clusters in photoreceptor terminals.

(J–L) Adult brain immunolabeled with TOTO3 to mark nuclei (blue), with nc82 to mark active zones (green), and for NMNAT (red). NMNAT is expressed at higher levels in neuronal nuclei (marked by TOTO3) in the brain and lower levels in the neuropil (marked by nc82). AL, antenna lobe; MB, mushroom body.

Scale bars indicate 5 μm.