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An infectious HERV-K(HML2) retrovirus can be generated by in vitro recombination from cloned HERV-K loci. Structure of the cloned HERV-K(HML2) proviruses and of the in vitro constructed chimera (see Methods), with the RT activities in the supernatant of 293T cells transfected with the corresponding plasmids measured as in Figure 3C, and the associated infection efficiencies measured by quantitative PCR on the target cell genomic DNA (BHK21 cells, see Methods).
