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Effects of Tb on TAG content and biosynthesis
| TAG | Tb Concentration
|
|||
|---|---|---|---|---|
| 0 | 3 | 15 | 30 | |
| μm | ||||
| μmol g−1 dwt | 0.5 ± 0.1 | 1.6 ± 0.2 | ND | 4.1 ± 0.6 |
| cpm g−1 dwt × 103 | 21 | 73 | 92 | 118 |
Three-day-old TBY-2 cells were treated for 24 h with different concentrations (from 0 to 30 μm) of Tb, then incubated with sodium [1-14C]acetate for 2 h just before cell harvesting. TAGs were extracted and quantified as described in “Materials and Methods.” ND, Not determined; dwt, dry weight.
