Figure 8.

Two-day-old seedlings of peanuts were grown in the dark at 25°C, and were treated with 250 mm NaCl at 25°C and harvested after the indicated time. A, Time course of mRNA expression of salt-treated seedlings. B, Densitometry scan of relative mRNA levels obtained from Figure 8A. Error bars represent the sd of two independent experiments. C, Immunoblot of salt-treated seedlings with STY kinase antibodies at the indicated time periods. D, Protein extracts (250 μg) from untreated control (0 min) or seedlings treated with cold (4°C) were immunoprecipitated with 1:100 (v/v) dilution of anti-STY kinase antibodies. Kinase activity of the immune complex was subsequently determined using histone III-S as an exogenous substrate.