Figure 2.

Suc inhibits the loss of nuclear gene expression in FRc-grown seedlings transferred to WL. A, Greening of cotyledons under different WL fluence rates after growth in darkness or FRc. Seedlings were germinated on 3% (w/v) Suc in the dark for 2 d. They were subsequently grown for a further period (as indicated) under FRc (white symbols) or were maintained in darkness (black symbols) for the equivalent period. Seedlings were then transferred to a WL source of 250 μmol m⁻² s⁻¹ (circles) or 8 μmol m⁻² s⁻¹ (triangles). B, HEMA1 and Lhcb mRNA accumulation under WL (130 μmol m⁻² s⁻¹) following a 1- or 3-d FRc pretreatment. Seedlings were grown on 3% (w/v) Suc. The RNA gel blot was sequentially hybridized with probes for HEMA1, Lhcb, and 18S transcripts.