Figure 1.

Immunoblot analysis of the light stabilities of the five Arabidopsis phytochromes. Seedlings were grown for 7 d in continuous darkness (D) or WL, or in darkness and then placed under R for 3 h (R₃), 12 h (R₁₂), or 24 h (R₂₄) before harvest at 7 d. Extracts were prepared and precipitated with 25% (w/v) (NH₄)₂SO₄ for phyA, B, C, and E or with 20% (w/v) (NH₄)₂SO₄ for phyD. An amount of protein equivalent to 300 μg of total extractable protein was loaded in the D, R₃, R₁₂, and R₂₄ lanes and an amount equivalent to 390 μg in the WL lane. For each phytochrome, dilution curves of the darkness extract were included to estimate the -fold difference in the level of that apoprotein under the different light conditions. MAbs used were: phyA, 073d; phyB, B6B3; phyC, C11 and C13; phyD, 2C1; and phyE, 7B3.