Figure 5.

RNA gel-blot analysis of the relative abundance of BEBT mRNA transcripts in the stigma, stamen, style, sepals, petals, and leaves of C. breweri plants. Tissues were harvested from mature plants and floral tissue samples were taken from flowers on d 1 of anthesis. Lanes were loaded with 4 μg of total RNA. After probing with the BEBT probe and quantitation of the results, each blot was rehybridized with an 18S rDNA probe to normalize samples. A sample blot is shown above. The graphical representation below represents an average of three independent experiments.