Figure 1.

Effect of IL-1 on bone resorption in BM cocultures in WT and iNOS KO mice. Recombinant human IL-1β (10 units/ml) or vehicle was added to the cocultures at day 7, and the cultures were terminated on day 10. Columns are means and bars are SD of six replicates. (a) Resorption surface area. (b) Number of osteoclasts per dentine slice. (c) Nitrite levels of the cultures at day 10. (d) Resorption area per osteoclast. OB, osteoblasts; OC, osteoclasts; KO, iNOS KO. *, P < 0.05 from vehicle; **, P < 0.01; ***, P < 0.001. The results shown are a representative experiment of three performed. Basal OC numbers and resorption levels in the other two experiments were: 125 ± 26 OC per well and 0.31 mm², and 278 ± 35 OC per well and 0.58 mm² for WT cocultures; and 212 ± 13 OC per well and 0.42 mm², and 263 ± 35 OC per well and 0.69 mm² for KO cocultures.